Virus:Article Title: A single vomeronasal receptor promotes intermale aggression through dedicated hypothalamic neurons.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies pS6 Ribosomal Protein (S235/236) Rabbit mAb Cell Signaling Technology Cat# 4856S; RRID:AB_2181037 Alexa488-conjugated goat anti-rabbit antibody Invitrogen Cat#A11034; RRID:AB_2576217 Alexa488-conjugated goat anti-chicken antibody Invitrogen Cat#A11039; RRID:AB_142924 Horseradish peroxidase (HRP)-conjugated anti-Dig antibody Roche Applied Science Cat# 11207733910; RRID:AB_514500 Horseradish peroxidase (HRP)-conjugated anti-Flu antibody Roche Applied Science Cat#11426346910; RRID:AB_840257 Chicken anti-GFP antibody Aves Labs Cat#GFP-1020; RRID:AB_10000240 Bacterial and virus strains AAV serotype 5 CAG-FLEx-GCAMP6s Addgene (Chen et al., 2013) Addgene number: 100842; RRID:Addgene_100842 AAV serotype 8 CAG-FLEx-GFP UNC Vector Core AV4910B AAV serotype 8 hSyn-DIO-hM4D(Gi)-mCherry Addgene (Krashes et al., 2011) Addgene number: 44362; RRID:Addgene_44362 AAV serotype 8 hSyn-DIO-hM3D(Gq)-mCherry Addgene (Krashes et al., 2011) Addgene number: 44361; RRID:Addgene_44361 AAV serotype 8 hSyn-DIO-mCherry Addgene Addgene number: 50459; RRID:Addgene_50459 AAV serotype 2 CAG-FLEx-TCb Miyamichi et al. (2013) (Custom made by UNC Vector Core) N/A AAV serotype 2 CAG-FLEx-RG Miyamichi et al., 2013 (Custom made by UNC Vector Core) N/A Rabies dG-GFP+EnvA Amplified in the lab from a virus shared by Dr. Masahiro Yamaguchi N/A Chemicals, peptides, and recombinant proteins TSA-plus Cyanine3 Akoya Bioscience Cat#NEL744001KT TSA-plus biotin Akoya Bioscience Cat#NEL749A001KT Streptavidin-Alexa Fluor 488 Life Technologies Cat#S32354 Clozapine N-oxide (CNO) Sigma-Aldrich Cat#C0832-5MG Experimental models: Cell lines B7GG cell line Gift from Dr. Edward Callaway Osakada and Callaway, 2013 BHK-EnvA cell line Gift from Dr. Edward Callaway Osakada and Callaway, 2013 HEK293-TVA800 cell line Gift from Dr. Edward Callaway Osakada and Callaway, 2013 Experimental models: Organisms/strains Mouse: Vmn2r53-deficient This paper N/A Mouse: PR-Cre (B6.129S(Cg)-Pgrtm1.1(cre) Shah/AndJ) JAX Laboratories JAX#017915;RRID:IMSR_JAX:017915 Mouse: C57BL/6JJmsSlc Japan SLC N/A Mouse: BALB/cCrSlc Japan SLC N/A Mouse: C3H/HeSlc Japan SLC N/A Mouse: DBA/1JJmsSlc Japan SLC N/A Mouse: DBA/2CrSlc Japan SLC N/A Mouse: A/JJmsSlc Japan SLC N/A Mouse: CBA/NSlc Japan SLC N/A Mouse: 129X1/SvJJmsSlc Japan SLC N/A Mouse: Slc:ICR Japan SLC N/A (Continued on next page) e1 Neuron 110, 2455–2469.e1–e8, August 3, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides Primers for RNA probes, see TableS1 This paper N/A Primers for generation and genotypes of Vmn2r53-deficient mice, see method details This paper N/A Recombinant DNA Plasmid: ESP1 Kimoto et al., 2005 N/A Plasmid: ESP22 Ferrero et al., 2013 N/A Plasmid: MUP3 Kaur et al., 2014 N/A Plasmid: MUP7 This paper N/A Plasmid: MUP19 This paper N/A Software and algorithms Python v3 Python Software Foundation http://www.python.org/ ImageJ NIH https://imagej.nih.gov/ij MATLAB MathWorks https://jp.mathworks.com/products/matlab.html Other AMICON ULTRA-15 15ML - 3 KDa cutoff Millipore Cat#UFC900324 Strata-X Polymer Reversed Phase 33 mm, 500 mg/6 mL Phenomenex Cat#8B-S100-HCH Discovery DSC NH2, 12 mL Tube, 2 gram Spelco Cat#52641-U ll
Bioprocessing:Article Title: A single vomeronasal receptor promotes intermale aggression through dedicated hypothalamic neurons.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies pS6 Ribosomal Protein (S235/236) Rabbit mAb Cell Signaling Technology Cat# 4856S; RRID:AB_2181037 Alexa488-conjugated goat anti-rabbit antibody Invitrogen Cat#A11034; RRID:AB_2576217 Alexa488-conjugated goat anti-chicken antibody Invitrogen Cat#A11039; RRID:AB_142924 Horseradish peroxidase (HRP)-conjugated anti-Dig antibody Roche Applied Science Cat# 11207733910; RRID:AB_514500 Horseradish peroxidase (HRP)-conjugated anti-Flu antibody Roche Applied Science Cat#11426346910; RRID:AB_840257 Chicken anti-GFP antibody Aves Labs Cat#GFP-1020; RRID:AB_10000240 Bacterial and virus strains AAV serotype 5 CAG-FLEx-GCAMP6s Addgene (Chen et al., 2013) Addgene number: 100842; RRID:Addgene_100842 AAV serotype 8 CAG-FLEx-GFP UNC Vector Core AV4910B AAV serotype 8 hSyn-DIO-hM4D(Gi)-mCherry Addgene (Krashes et al., 2011) Addgene number: 44362; RRID:Addgene_44362 AAV serotype 8 hSyn-DIO-hM3D(Gq)-mCherry Addgene (Krashes et al., 2011) Addgene number: 44361; RRID:Addgene_44361 AAV serotype 8 hSyn-DIO-mCherry Addgene Addgene number: 50459; RRID:Addgene_50459 AAV serotype 2 CAG-FLEx-TCb Miyamichi et al. (2013) (Custom made by UNC Vector Core) N/A AAV serotype 2 CAG-FLEx-RG Miyamichi et al., 2013 (Custom made by UNC Vector Core) N/A Rabies dG-GFP+EnvA Amplified in the lab from a virus shared by Dr. Masahiro Yamaguchi N/A Chemicals, peptides, and recombinant proteins TSA-plus Cyanine3 Akoya Bioscience Cat#NEL744001KT TSA-plus biotin Akoya Bioscience Cat#NEL749A001KT Streptavidin-Alexa Fluor 488 Life Technologies Cat#S32354 Clozapine N-oxide (CNO) Sigma-Aldrich Cat#C0832-5MG Experimental models: Cell lines B7GG cell line Gift from Dr. Edward Callaway Osakada and Callaway, 2013 BHK-EnvA cell line Gift from Dr. Edward Callaway Osakada and Callaway, 2013 HEK293-TVA800 cell line Gift from Dr. Edward Callaway Osakada and Callaway, 2013 Experimental models: Organisms/strains Mouse: Vmn2r53-deficient This paper N/A Mouse: PR-Cre (B6.129S(Cg)-Pgrtm1.1(cre) Shah/AndJ) JAX Laboratories JAX#017915;RRID:IMSR_JAX:017915 Mouse: C57BL/6JJmsSlc Japan SLC N/A Mouse: BALB/cCrSlc Japan SLC N/A Mouse: C3H/HeSlc Japan SLC N/A Mouse: DBA/1JJmsSlc Japan SLC N/A Mouse: DBA/2CrSlc Japan SLC N/A Mouse: A/JJmsSlc Japan SLC N/A Mouse: CBA/NSlc Japan SLC N/A Mouse: 129X1/SvJJmsSlc Japan SLC N/A Mouse: Slc:ICR Japan SLC N/A (Continued on next page) e1 Neuron 110, 2455–2469.e1–e8, August 3, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides Primers for RNA probes, see TableS1 This paper N/A Primers for generation and genotypes of Vmn2r53-deficient mice, see method details This paper N/A Recombinant DNA Plasmid: ESP1 Kimoto et al., 2005 N/A Plasmid: ESP22 Ferrero et al., 2013 N/A Plasmid: MUP3 Kaur et al., 2014 N/A Plasmid: MUP7 This paper N/A Plasmid: MUP19 This paper N/A Software and algorithms Python v3 Python Software Foundation http://www.python.org/ ImageJ NIH https://imagej.nih.gov/ij MATLAB MathWorks https://jp.mathworks.com/products/matlab.html Other AMICON ULTRA-15 15ML - 3 KDa cutoff Millipore Cat#UFC900324 Strata-X Polymer Reversed Phase 33 mm, 500 mg/6 mL Phenomenex Cat#8B-S100-HCH Discovery DSC NH2, 12 mL Tube, 2 gram Spelco Cat#52641-U ll
Article Title: BAR-Seq clonal tracking of gene-edited cells.
Article Snippet: .. Plasmids required for AAV production are commercially available (e.g., the AAV genome by Agilent Technologies (cat. no. 240071) and the pDGM plasmid for rep-cap and helper expression by Addgene/Russell’s laboratory (Addgene number 110660 for AAV2/6)). ..
Amplification:Article Title: A single vomeronasal receptor promotes intermale aggression through dedicated hypothalamic neurons.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies pS6 Ribosomal Protein (S235/236) Rabbit mAb Cell Signaling Technology Cat# 4856S; RRID:AB_2181037 Alexa488-conjugated goat anti-rabbit antibody Invitrogen Cat#A11034; RRID:AB_2576217 Alexa488-conjugated goat anti-chicken antibody Invitrogen Cat#A11039; RRID:AB_142924 Horseradish peroxidase (HRP)-conjugated anti-Dig antibody Roche Applied Science Cat# 11207733910; RRID:AB_514500 Horseradish peroxidase (HRP)-conjugated anti-Flu antibody Roche Applied Science Cat#11426346910; RRID:AB_840257 Chicken anti-GFP antibody Aves Labs Cat#GFP-1020; RRID:AB_10000240 Bacterial and virus strains AAV serotype 5 CAG-FLEx-GCAMP6s Addgene (Chen et al., 2013) Addgene number: 100842; RRID:Addgene_100842 AAV serotype 8 CAG-FLEx-GFP UNC Vector Core AV4910B AAV serotype 8 hSyn-DIO-hM4D(Gi)-mCherry Addgene (Krashes et al., 2011) Addgene number: 44362; RRID:Addgene_44362 AAV serotype 8 hSyn-DIO-hM3D(Gq)-mCherry Addgene (Krashes et al., 2011) Addgene number: 44361; RRID:Addgene_44361 AAV serotype 8 hSyn-DIO-mCherry Addgene Addgene number: 50459; RRID:Addgene_50459 AAV serotype 2 CAG-FLEx-TCb Miyamichi et al. (2013) (Custom made by UNC Vector Core) N/A AAV serotype 2 CAG-FLEx-RG Miyamichi et al., 2013 (Custom made by UNC Vector Core) N/A Rabies dG-GFP+EnvA Amplified in the lab from a virus shared by Dr. Masahiro Yamaguchi N/A Chemicals, peptides, and recombinant proteins TSA-plus Cyanine3 Akoya Bioscience Cat#NEL744001KT TSA-plus biotin Akoya Bioscience Cat#NEL749A001KT Streptavidin-Alexa Fluor 488 Life Technologies Cat#S32354 Clozapine N-oxide (CNO) Sigma-Aldrich Cat#C0832-5MG Experimental models: Cell lines B7GG cell line Gift from Dr. Edward Callaway Osakada and Callaway, 2013 BHK-EnvA cell line Gift from Dr. Edward Callaway Osakada and Callaway, 2013 HEK293-TVA800 cell line Gift from Dr. Edward Callaway Osakada and Callaway, 2013 Experimental models: Organisms/strains Mouse: Vmn2r53-deficient This paper N/A Mouse: PR-Cre (B6.129S(Cg)-Pgrtm1.1(cre) Shah/AndJ) JAX Laboratories JAX#017915;RRID:IMSR_JAX:017915 Mouse: C57BL/6JJmsSlc Japan SLC N/A Mouse: BALB/cCrSlc Japan SLC N/A Mouse: C3H/HeSlc Japan SLC N/A Mouse: DBA/1JJmsSlc Japan SLC N/A Mouse: DBA/2CrSlc Japan SLC N/A Mouse: A/JJmsSlc Japan SLC N/A Mouse: CBA/NSlc Japan SLC N/A Mouse: 129X1/SvJJmsSlc Japan SLC N/A Mouse: Slc:ICR Japan SLC N/A (Continued on next page) e1 Neuron 110, 2455–2469.e1–e8, August 3, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides Primers for RNA probes, see TableS1 This paper N/A Primers for generation and genotypes of Vmn2r53-deficient mice, see method details This paper N/A Recombinant DNA Plasmid: ESP1 Kimoto et al., 2005 N/A Plasmid: ESP22 Ferrero et al., 2013 N/A Plasmid: MUP3 Kaur et al., 2014 N/A Plasmid: MUP7 This paper N/A Plasmid: MUP19 This paper N/A Software and algorithms Python v3 Python Software Foundation http://www.python.org/ ImageJ NIH https://imagej.nih.gov/ij MATLAB MathWorks https://jp.mathworks.com/products/matlab.html Other AMICON ULTRA-15 15ML - 3 KDa cutoff Millipore Cat#UFC900324 Strata-X Polymer Reversed Phase 33 mm, 500 mg/6 mL Phenomenex Cat#8B-S100-HCH Discovery DSC NH2, 12 mL Tube, 2 gram Spelco Cat#52641-U ll
Article Title: Mapping the transcriptional and epigenetic landscape of organotypic endothelial diversity in the developing and adult mouse.
Article Snippet: The vascular endothelium features unique molecular and functional properties across different vessel types, such as between arteries, veins and capillaries, as well as between different organs, such as the leaky sinusoidal endothelium of the liver versus the impermeable vessels of the brain.. However, the transcriptional networks governing endothelial organ specialization remain unclear.. Here we profile the accessible chromatin and transcriptional landscapes of the endothelium from the mouse liver, lung, heart, kidney, brain and retina, across developmental time, to identify potential transcriptional regulators of endothelial heterogeneity.
Recombinant:Article Title: A single vomeronasal receptor promotes intermale aggression through dedicated hypothalamic neurons.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies pS6 Ribosomal Protein (S235/236) Rabbit mAb Cell Signaling Technology Cat# 4856S; RRID:AB_2181037 Alexa488-conjugated goat anti-rabbit antibody Invitrogen Cat#A11034; RRID:AB_2576217 Alexa488-conjugated goat anti-chicken antibody Invitrogen Cat#A11039; RRID:AB_142924 Horseradish peroxidase (HRP)-conjugated anti-Dig antibody Roche Applied Science Cat# 11207733910; RRID:AB_514500 Horseradish peroxidase (HRP)-conjugated anti-Flu antibody Roche Applied Science Cat#11426346910; RRID:AB_840257 Chicken anti-GFP antibody Aves Labs Cat#GFP-1020; RRID:AB_10000240 Bacterial and virus strains AAV serotype 5 CAG-FLEx-GCAMP6s Addgene (Chen et al., 2013) Addgene number: 100842; RRID:Addgene_100842 AAV serotype 8 CAG-FLEx-GFP UNC Vector Core AV4910B AAV serotype 8 hSyn-DIO-hM4D(Gi)-mCherry Addgene (Krashes et al., 2011) Addgene number: 44362; RRID:Addgene_44362 AAV serotype 8 hSyn-DIO-hM3D(Gq)-mCherry Addgene (Krashes et al., 2011) Addgene number: 44361; RRID:Addgene_44361 AAV serotype 8 hSyn-DIO-mCherry Addgene Addgene number: 50459; RRID:Addgene_50459 AAV serotype 2 CAG-FLEx-TCb Miyamichi et al. (2013) (Custom made by UNC Vector Core) N/A AAV serotype 2 CAG-FLEx-RG Miyamichi et al., 2013 (Custom made by UNC Vector Core) N/A Rabies dG-GFP+EnvA Amplified in the lab from a virus shared by Dr. Masahiro Yamaguchi N/A Chemicals, peptides, and recombinant proteins TSA-plus Cyanine3 Akoya Bioscience Cat#NEL744001KT TSA-plus biotin Akoya Bioscience Cat#NEL749A001KT Streptavidin-Alexa Fluor 488 Life Technologies Cat#S32354 Clozapine N-oxide (CNO) Sigma-Aldrich Cat#C0832-5MG Experimental models: Cell lines B7GG cell line Gift from Dr. Edward Callaway Osakada and Callaway, 2013 BHK-EnvA cell line Gift from Dr. Edward Callaway Osakada and Callaway, 2013 HEK293-TVA800 cell line Gift from Dr. Edward Callaway Osakada and Callaway, 2013 Experimental models: Organisms/strains Mouse: Vmn2r53-deficient This paper N/A Mouse: PR-Cre (B6.129S(Cg)-Pgrtm1.1(cre) Shah/AndJ) JAX Laboratories JAX#017915;RRID:IMSR_JAX:017915 Mouse: C57BL/6JJmsSlc Japan SLC N/A Mouse: BALB/cCrSlc Japan SLC N/A Mouse: C3H/HeSlc Japan SLC N/A Mouse: DBA/1JJmsSlc Japan SLC N/A Mouse: DBA/2CrSlc Japan SLC N/A Mouse: A/JJmsSlc Japan SLC N/A Mouse: CBA/NSlc Japan SLC N/A Mouse: 129X1/SvJJmsSlc Japan SLC N/A Mouse: Slc:ICR Japan SLC N/A (Continued on next page) e1 Neuron 110, 2455–2469.e1–e8, August 3, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides Primers for RNA probes, see TableS1 This paper N/A Primers for generation and genotypes of Vmn2r53-deficient mice, see method details This paper N/A Recombinant DNA Plasmid: ESP1 Kimoto et al., 2005 N/A Plasmid: ESP22 Ferrero et al., 2013 N/A Plasmid: MUP3 Kaur et al., 2014 N/A Plasmid: MUP7 This paper N/A Plasmid: MUP19 This paper N/A Software and algorithms Python v3 Python Software Foundation http://www.python.org/ ImageJ NIH https://imagej.nih.gov/ij MATLAB MathWorks https://jp.mathworks.com/products/matlab.html Other AMICON ULTRA-15 15ML - 3 KDa cutoff Millipore Cat#UFC900324 Strata-X Polymer Reversed Phase 33 mm, 500 mg/6 mL Phenomenex Cat#8B-S100-HCH Discovery DSC NH2, 12 mL Tube, 2 gram Spelco Cat#52641-U ll
Crocin Bleaching Assay:Article Title: A single vomeronasal receptor promotes intermale aggression through dedicated hypothalamic neurons.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies pS6 Ribosomal Protein (S235/236) Rabbit mAb Cell Signaling Technology Cat# 4856S; RRID:AB_2181037 Alexa488-conjugated goat anti-rabbit antibody Invitrogen Cat#A11034; RRID:AB_2576217 Alexa488-conjugated goat anti-chicken antibody Invitrogen Cat#A11039; RRID:AB_142924 Horseradish peroxidase (HRP)-conjugated anti-Dig antibody Roche Applied Science Cat# 11207733910; RRID:AB_514500 Horseradish peroxidase (HRP)-conjugated anti-Flu antibody Roche Applied Science Cat#11426346910; RRID:AB_840257 Chicken anti-GFP antibody Aves Labs Cat#GFP-1020; RRID:AB_10000240 Bacterial and virus strains AAV serotype 5 CAG-FLEx-GCAMP6s Addgene (Chen et al., 2013) Addgene number: 100842; RRID:Addgene_100842 AAV serotype 8 CAG-FLEx-GFP UNC Vector Core AV4910B AAV serotype 8 hSyn-DIO-hM4D(Gi)-mCherry Addgene (Krashes et al., 2011) Addgene number: 44362; RRID:Addgene_44362 AAV serotype 8 hSyn-DIO-hM3D(Gq)-mCherry Addgene (Krashes et al., 2011) Addgene number: 44361; RRID:Addgene_44361 AAV serotype 8 hSyn-DIO-mCherry Addgene Addgene number: 50459; RRID:Addgene_50459 AAV serotype 2 CAG-FLEx-TCb Miyamichi et al. (2013) (Custom made by UNC Vector Core) N/A AAV serotype 2 CAG-FLEx-RG Miyamichi et al., 2013 (Custom made by UNC Vector Core) N/A Rabies dG-GFP+EnvA Amplified in the lab from a virus shared by Dr. Masahiro Yamaguchi N/A Chemicals, peptides, and recombinant proteins TSA-plus Cyanine3 Akoya Bioscience Cat#NEL744001KT TSA-plus biotin Akoya Bioscience Cat#NEL749A001KT Streptavidin-Alexa Fluor 488 Life Technologies Cat#S32354 Clozapine N-oxide (CNO) Sigma-Aldrich Cat#C0832-5MG Experimental models: Cell lines B7GG cell line Gift from Dr. Edward Callaway Osakada and Callaway, 2013 BHK-EnvA cell line Gift from Dr. Edward Callaway Osakada and Callaway, 2013 HEK293-TVA800 cell line Gift from Dr. Edward Callaway Osakada and Callaway, 2013 Experimental models: Organisms/strains Mouse: Vmn2r53-deficient This paper N/A Mouse: PR-Cre (B6.129S(Cg)-Pgrtm1.1(cre) Shah/AndJ) JAX Laboratories JAX#017915;RRID:IMSR_JAX:017915 Mouse: C57BL/6JJmsSlc Japan SLC N/A Mouse: BALB/cCrSlc Japan SLC N/A Mouse: C3H/HeSlc Japan SLC N/A Mouse: DBA/1JJmsSlc Japan SLC N/A Mouse: DBA/2CrSlc Japan SLC N/A Mouse: A/JJmsSlc Japan SLC N/A Mouse: CBA/NSlc Japan SLC N/A Mouse: 129X1/SvJJmsSlc Japan SLC N/A Mouse: Slc:ICR Japan SLC N/A (Continued on next page) e1 Neuron 110, 2455–2469.e1–e8, August 3, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides Primers for RNA probes, see TableS1 This paper N/A Primers for generation and genotypes of Vmn2r53-deficient mice, see method details This paper N/A Recombinant DNA Plasmid: ESP1 Kimoto et al., 2005 N/A Plasmid: ESP22 Ferrero et al., 2013 N/A Plasmid: MUP3 Kaur et al., 2014 N/A Plasmid: MUP7 This paper N/A Plasmid: MUP19 This paper N/A Software and algorithms Python v3 Python Software Foundation http://www.python.org/ ImageJ NIH https://imagej.nih.gov/ij MATLAB MathWorks https://jp.mathworks.com/products/matlab.html Other AMICON ULTRA-15 15ML - 3 KDa cutoff Millipore Cat#UFC900324 Strata-X Polymer Reversed Phase 33 mm, 500 mg/6 mL Phenomenex Cat#8B-S100-HCH Discovery DSC NH2, 12 mL Tube, 2 gram Spelco Cat#52641-U ll
Plasmid Preparation:Article Title: BAR-Seq clonal tracking of gene-edited cells.
Article Snippet: .. Plasmids required for AAV production are commercially available (e.g., the AAV genome by Agilent Technologies (cat. no. 240071) and the pDGM plasmid for rep-cap and helper expression by Addgene/Russell’s laboratory (Addgene number 110660 for AAV2/6)). ..
Expressing:Article Title: BAR-Seq clonal tracking of gene-edited cells.
Article Snippet: .. Plasmids required for AAV production are commercially available (e.g., the AAV genome by Agilent Technologies (cat. no. 240071) and the pDGM plasmid for rep-cap and helper expression by Addgene/Russell’s laboratory (Addgene number 110660 for AAV2/6)). ..
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